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Identification of layer-specific markers of hippocampal neurons

Deep CA1 pyramidal cell from the rat hippocampus.

Deep CA1 pyramidal cell from the rat hippocampus.

The hippocampus exhibits a huge genetic heterogeneity not only along the septo-temporal axis, but regionally from different subdivisions of CA1, CA2 and CA3 and from deep to superficial locations along the cornu ammonis. These genetic gradients link to differential contribution of hippocampal regions to cognitive processes such as episodic and spatial memory, and in hippocampal-specific diseases such as temporal lobe epilepsy. Hence, we adopted a genetic dissection approach of hippocampal regions by focusing on the laminar heterogeneity (deep-superficial) along the cornu ammonis (proximo-distal). Using RNAseq, in situ hybridization, immunostaining and electrophysiology we aim to recognize and validate genes regionalized in deep and superficial layers and to identify hippocampal cellular populations specifically vulnerable to epilepsy.